IF=14.2994，Advanced Science，中國(guó)藥科大學(xué)，AAV
"Genomeditech Biotechnology (Shanghai, China) constructed the adenoviruses. To specifically knock down Fis1 protein expression in the endothelium of ApoE?/? mice, adeno-associated virus serotype 9 (AAV9) carrying Fis1 shRNA driven by the endothelial-specific Tie2 promoter was used. The Fis1 shRNA targeting sequence was 5′CCTGATTGATAAGGCCATGAA-3′, and the control shRNA sequence was 5′-TTCTCCGAACGTGTCACGT-3′. tie2干擾AAV，內(nèi)皮"
IF=14.2994，Advanced Science，上海交通大學(xué)醫(yī)學(xué)院附屬瑞金醫(yī)院，質(zhì)粒；轉(zhuǎn)染試劑
" The vector control and PLXDC1 expression plasmids were produced by Genomeditech (Shanghai, China). The human PSCs cell line was transfected with either the vector control or PLXDC1 expression plasmid using GMTrans Liposomal Transfection Reagent (Genomeditech, Shanghai, China)"
IF=14.2994，Advanced Science，南京醫(yī)科大學(xué)第一附屬醫(yī)院，質(zhì)粒
" The short hair RNAs (shRNAs) of TRIM38 and CCT6AweresynthesizedbyGenomeditech(Shanghai,China).Theoverex pression plasmids, including Flag-TRIM38, His-CCT6A, and HA-ubiquitin, were obtained from Genomeditech. "
IF=14.6001，Autophagy，上海交通大學(xué)醫(yī)學(xué)院附屬胸科醫(yī)院，質(zhì)粒，熒光素酶檢測(cè)試劑盒
The sequence of the LAMP2 promoter was inserted into the pGL3-Basic vector (Genomeditech, GM-1013FL01). Cells were transfected with the constructed pGL3-Basic-LAMP2 plasmid, together with STAT3 overexpression or empty vector plasmids. Following 48?h of co-transfection with the pRL-TK-Renilla luciferase plasmid, luciferase activity was detected and quantified using the Dual-Luciferase Kit (Genomeditech, GM-040503A) under the manufacturer’s instruction. All experiments were repeated three times.
